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Part:BBa_K2132099:Design

Designed by: YIFAN CHEN   Group: iGEM16_ShanghaitechChina   (2016-10-09)


HydA with SpyCatcher, Histag and TEV site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used Histag for protein purification and added the TEV site so that it will be easy to cut off the attached Histag.


Source

Clostridium acetobutylicum ATCC 824.

References